Learning Wednesday
Practical insights on 3D tissue imaging and spatial biology.
A weekly LinkedIn series from Alpenglow Biosciences, collected here for easy access to research papers, concepts, and practical examples in 3D tissue imaging, digital pathology, spatial profiling, volumetric datasets, and AI-powered analysis.
Nuclear morphology and 3D imaging for cellular neighborhoods
A Learning Wednesday paper note on 3D nuclear morphology and cellular neighborhood profiling.
Tumour evolution and microenvironment interactions in 2D and 3D space
A Learning Wednesday paper note on tumour evolution and microenvironment interactions across 2D and 3D space.
Why three-dimensional tissue assessment matters
A Learning Wednesday paper note on why 3D assessment changes tissue composition measurements.
3D histology of immune response to pancreatic precancers
A Learning Wednesday paper note on 3D histology of heterogeneous immune responses in pancreatic precancers.
3D imaging of nerves, glia, and mast cells in human sigmoid mucosal biopsies
A Learning Wednesday paper note on 3D quantitation of nerves, glia, mast cells, and spatial proximity in gut biopsies.
3D spatial transcriptomics and ECM imaging in lung cancer
A Learning Wednesday paper note on AI-enabled 3D spatial transcriptomics.
3D imaging for driving cancer discovery
A Learning Wednesday paper note on how 3D imaging methods are changing cancer discovery.
Highly multiplexed 3D profiling of immune niches in human tumours
A Learning Wednesday paper note on multiplexed 3D profiling of immune niches in human tumors.
3D imaging of keloid vasculature
A Learning Wednesday paper note on 3D imaging of abnormal vasculature in keloid tissue.
3D spatial transcriptomics and ECM imaging in the tumor microenvironment
A Learning Wednesday paper note on AI-enabled 3D spatial transcriptomics.
3D optical clearing reveals epidermal innervation in atopic dermatitis and psoriasis
A Learning Wednesday paper note on 3D optical clearing and epidermal innervation in inflammatory skin disease.
3D clonal mapping in normal endometrial epithelium
A Learning Wednesday paper note on spatial clonal dynamics in normal endometrial epithelium.
X-Pression: deep learning-based 3D spatial transcriptomics
A Learning Wednesday paper note on AI-enabled 3D spatial transcriptomics.
Hybrid open-top light sheet microscopy for cleared tissues
A Learning Wednesday paper note on hybrid open-top light sheet microscopy for multiscale cleared tissue imaging.
VORTEX: AI-driven 3D spatial transcriptomics
A Learning Wednesday paper note on AI-enabled 3D spatial transcriptomics.
Cleared human melanoma analyzed in 3D
A Learning Wednesday paper note on 3D analysis of cleared human melanoma tissue.
3D imaging of cleared human skin biopsies with light sheet microscopy
A Learning Wednesday paper note on light sheet microscopy for full-depth skin biopsy visualization.
Skin-iDISCO+ for 3D analysis of human cutaneous vasculature
A Learning Wednesday paper note on tissue clearing and 3D analysis of human cutaneous vasculature.
3D imaging of upper tract urothelial carcinoma
A Learning Wednesday paper note on 3D pathology approaches for upper tract urothelial carcinoma diagnosis.
Harnessing non-destructive 3D pathology
A Learning Wednesday paper note on digital pathology.