Research from Alpenglow Biosciences and collaborators.

Device publications on open-top light-sheet microscopy and 3Di HOTLS

1. Assessing the effects of a 3D pathology tissue-processing workflow on downstream molecular analyses | bioRxiv [Preprint] 2026 | Link

2. A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues | Nature Methods 2022 | Link

3. Harnessing non-destructive 3D pathology | Nature Biomedical Engineering | Feb 15, 2021 | Link

4. Multi-immersion open-top light-sheet microscope for high-throughput imaging of cleared tissues | Nature Communications | Jul 4, 2019 | Link

5. Light-sheet microscopy for slide-free non-destructive pathology of large clinical specimens | Nature Biomedical Engineering | Jun 26, 2017 | Link

Clinical and translational studies using 3D pathology and OTLS

1. 3D pathology-guided microdissection | bioRxiv [Preprint] 2025 | Link. 

2. Deep-learning triage of 3D pathology datasets for comprehensive and efficient pathologist assessments | bioRxiv [Preprint] 2025 | Link

3. Analysis of 3D pathology samples using weakly supervised AI | Cell May 2024| Link

4. Ablating Lgr5-expressing prostatic stromal cells activates the ERK-mediated mechanosensory signaling and disrupts prostate tissue homeostasis | Cell Rep. 2022 | Link

5. Prostate cancer risk stratification via non-destructive 3D pathology with deep learning-assisted gland analysis | Cancer Research | Nov 24, 2021 | Link

6. Diagnosing 12 prostate needle cores within an hour of biopsy via open-top light-sheet microscopy | Journal of Biomedical Optics | Dec 15, 2020 | Link

7. Open-top light-sheet (OTLS) fluorescence microscopy image atlas of prostate core-needle biopsies | Archives of Pathology & Laboratory Medicine | Sep 1, 2019 | Link

8. Rapid pathology of lumpectomy margins with open-top light-sheet (OTLS) microscopy | Biomedical Optics Express | Feb 19, 2019 | Link