3Di™: Hybrid Open-Top, Light-Sheet Microscope (HOTLS)
A dedicated instrument for high-resolution 3D tissue imaging
What the 3Di™ Open-Top Light-Sheet Microscope Does
The 3Di™ system converts intact biological specimens into quantitative 3D datasets.
It offers easy sample access, flexible sample holders, and two integrated optical paths for whole sample and high-resolution imaging.
Researchers can image large tissues, identify regions of interest, and acquire submicrometer detail within the same instrument.
With Aurora 3Di™, light-sheet microscopy becomes a seamless experience.
Open-Top Design Advantages
Easy Sample Access
The 3Di™ microscope's open-top design provides researchers with easy access to their samples. Multiple holders accommodate a wide range of shapes, sizes, and preparation methods.
Multiple Sample Scanning
The 3Di™ microscope supports multi-well and multi-specimen scanning.
This enables higher throughput when imaging multiple samples in a session.
Large Scan Area
The microscope's large scan area, up to 12 cm × 7.5 cm × 1 cm, enables the acquisition of complete 3D images of large biological tissues.
Internal Architecture & Sample Flexibility
A dedicated illumination path and two collection paths support precise acquisition.
The internal design maintains stable illumination, efficient light sheet delivery, and smooth switching between imaging modes.
Microscope Internal Design
Multiple sample holder configurations accommodate diverse specimen types and experimental requirements, ranging from organoids and small biopsies to thick tissue blocks and multiple whole organs loaded for simultaneous scanning.
Sample Holder Examples
Efficient Dual Optical Paths
Time Efficient
The system acquires overviews quickly in Scout mode, then captures high-resolution detail in Zoom mode, accelerating the imaging process and allowing researchers to obtain results faster.
Data Efficient
Scout and Zoom together provide a more complete representation of the tissue sample. Whole tissue context and local microstructure can be captured within a single workflow.
Versatile Imaging
The 3Di™ microscope provides a seamless transition between whole sample and high-resolution scans, allowing researchers to quickly analyze a sample at both low and high magnifications.
Advanced Objective Configuration
Illumination Objective
A dedicated objective is used to illuminate the sample with a sheet of light, providing high-quality optical sections and minimizing photobleaching.
Zoom Imaging
The Zoom (NODO) imaging mode provides high-resolution scans of regions of interest, enabling detailed analysis of specific areas.
Scout Imaging
The Scout (ODO) imaging mode provides a low-resolution, whole-sample scan, enabling rapid overview imaging and sample navigation.
3Di™: Dual Imaging Modes
Scout Mode
The Scout imaging mode provides rapid, low-resolution imaging, allowing for a quick sample overview and navigation.
Resolution: 2 μm/pixel
Zoom Mode
The Zoom imaging mode offers high-resolution imaging of specific regions of interest identified with the Scout mode, enabling detailed analysis.
Resolution: 0.17 μm/pixel
3Di™: Intuitive User Interface: LUMI
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Intuitive Scan Setup
The 3Di™ microscope's intuitive user interface streamlines the imaging process by simplifying scan setup
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Live Preview
The 3Di™ microscope provides simultaneous live previews in both Scout and Zoom modes, allowing for real-time visualization of your data.
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Easy Interface
The 3Di™ microscope's user-friendly interface allows researchers of all levels to navigate and control the system easily.
Exploring the Seamless LUMI Interface
Discover how LUMI's intelligent design makes complex 3D imaging workflows simple and efficient. The interface provides real-time control and visualization for both Scout and Zoom imaging modes.
See the power of dual imaging modes working together to deliver comprehensive sample analysis.
3D Tissue Imaging Datasets Generated with the 3Di™ HOTLS Microscope
3Di™ Technical Specifications
| Specification | Value |
|---|---|
| Format | Hybrid Open-Top Light-Sheet |
| Maximum Specimen Size | Up to 12 cm × 7.5 cm × 1 cm (x, y, z) |
| Maximum Collection | 0.7 NA |
| Maximum Illumination | 0.06 NA |
| Scout Resolution | 2 µm/pixel |
| Zoom Resolution | 0.17 µm/pixel |
| Low Power Resolution Magnification | 2.5 X |
| High Power Resolution Magnification | 40 X |
| Laser Lines (standard, now up to 5 channels) | 405 nm, 488 nm, 561 nm, 638 nm |
| Camera | 2 × Hamamatsu ORCA Fusion BT |
| Refractive Index Capability | RI 1.33 – 1.56 |
| Multi well, Multi-specimen Scanning | Yes |
| Microscope Control Software | LUMI Graphical User Interface |
Ready to Transform Your Research?
The 3Di™ microscope combines cutting-edge technology with a user-friendly design to deliver exceptional 3D imaging capabilities. From whole-sample overviews to high-resolution, detailed analysis, the 3Di™ provides the versatility and precision your research demands.