Immuno Oncology insights with the 3D I/O Pro™ Assay.

3D I/O Pro™ Assay:

Advanced Analysis of the Tumor Immune Microenvironment

The 3D I/O Pro™ Assay is a cutting-edge, end-to-end solution designed to analyze the Tumor Immune Microenvironment comprehensively. Our platform provides unprecedented insights into tumor classification, including distinctions between hot and cold tumors, immune exclusion patterns, collagen orientation, tertiary lymphoid structure (TLS) quantification, lymphocyte quantification, and spatial mapping of lymphocytes.

Key Benefits

  • Comprehensive, high-resolution 3D imaging of the tumor microenvironment.

  • Proprietary data analysis workflow for accurate and efficient results.

  • Flexible marker customization with a wide selection of pre-optimized options.

  • Accurate classification of tumors (hot vs. cold) and immune exclusion characterization.

  • Advanced lymphocyte quantification in 3D and spatial mapping capabilities.

  • Customizable marker panels for tailored research applications.

3D I/O Pro™ Assay:

The Workflow

The complete workflow begins with 3D imaging using Alpenglow Biosciences’ advanced 3Di Hybrid Open-Top Light Sheet Microscope, ensuring high-resolution imaging of tumor samples. The generated 3D data is then seamlessly managed and analyzed through our proprietary software, 3Dm, and 3Dai, which deliver precise, data-driven insights tailored to your research objectives.

3D I/O Pro™ Assay:

Markers selection

We provide flexibility and customization for marker selection.

Choose from a set of pre-optimized marker panels, or design your own by selecting from our established markers or having your preferred markers optimized.

Our platform supports easy marker swapping to accommodate the evolving needs of your experiments.

We offer optimized markers for multiple species, including humans and mice.

Option A

Select a pre-optimized panel

Option B

Design your panel

Unveil Tumor Biology in Three Dimensions

Identification of Memory T-Cells through CD45RO Staining in thick tissue.

Identification of B Cells and Follicular Dendritic Cells through CD21 Staining in thick tissue.