See 3D tissue imaging in motion.

A volumetric 3D view of human placenta tissue showing PD-L1 signal across the maternal fetal interface, revealing how immune related signals distribute through tissue architecture and depth.

3D fluorescence imaging of mouse skin reveals complex nerve branching across intact tissue, enabling quantitative spatial analysis beyond 2D histology.

High-resolution 3D imaging of nerve networks at the dermal–epidermal junction reveals complex branching and spatial organization across intact skin tissue.

This 3D video reveals the intricate branching architecture of peripheral nerves in mouse skin using Yo-Pro-1 and PGP9.5 staining, highlighting how 3D intact tissue imaging captures complex neural networks across the full tissue depth.

3D tissue imaging of mouse muscle reveals neural architecture across full volumetric context. NF200 labels large-caliber axons, PGP9.5 captures the broader neuronal network, and YO-PRO-1 marks nuclei, enabling quantitative spatial profiling and digital pathology workflows.

High-resolution 3D imaging of an intact mouse colorectal tumor reveals B and T cell organization and measurable tertiary lymphoid structures across the full tissue volume.

3D expansion microscopy of mouse kidney tissue reveals glomerular and extracellular matrix architecture with preserved volumetric context and enhanced spatial resolution.

High-resolution 3D imaging of an intact mouse colorectal tumor reveals B and T cell organization and tertiary lymphoid structures across the full tissue volume.

3D light-sheet imaging of an intact mouse colorectal tumor reveals B and T cells in their native spatial context, resolving single immune cells across the entire tissue volume.

High-resolution 3D imaging of atopic dermatitis reveals lymphocyte clusters near nerves, stained with TO-PRO-3, PGP 9.5, and CD45. Explore detailed innervation and immune-cell interactions in lesional skin.

3D imaging of cleared mouse fat pad reveals blood vessels, macrophages, and nerves labeled with lectin, CD68, and PGP9.5.

3D imaging of a whole murine heart stained with SMA reveals vascular structures and mural cells, from large vessels down to pericytes.

High-resolution 3D imaging of scalp tissue reveals entire follicles, nuclei, immune cells, and nerve networks, unlocking new insights into alopecia and other skin diseases.

FFPE colorectal tissue stained with YO-PRO-1 and anti-CD8 imaged in 3D at 2 μm/pixel on Aurora HOTLS. Quantification of CD8+ lymphocytes across whole section.

A volumetric 3D dataset of human colon tissue showing hallmark features of Hirschsprung’s disease. TO PRO3 and PGP9.5 staining highlights hypertrophic submucosal nerves alongside an absence of ganglion cells, providing a clear view of neural architecture within the GI tract.

3D imaging of skin reveals a hair follicle, its gland, and nerve network with stunning clarity, advancing research in alopecia and inflammatory skin disease.

3D imaging of the human placenta reveals vascular networks and cellular detail with a-SMA (green) and TO-PRO-3 (magenta). A striking view that advances maternal–fetal research.

Computational H&E staining, combined with 3D imaging, reveals the full structure of tertiary lymphoid structures (TLS) in NSCLC, overcoming 2D sampling bias and enabling the accurate quantification of volume, maturity, and spatial context.

Computational H&E staining combined with non-destructive 3D imaging reveals the true complexity of tertiary lymphoid structures (TLS) in NSCLC. Unlike thin 2D sections that risk misclassification, Alpenglow’s 3Di platform captures entire TLS morphology, volume, and cellular composition, delivering accurate insights into immune architecture and tumor context.

3D segmentation of prostate glands using synthetic CK8 immunofluorescence derived from fluorescent H&E analogues. By combining image-translation models with traditional computer-vision methods, researchers achieved whole-biopsy 3D gland segmentation without manual labeling.

3D imaging of melanoma tissue with CD45, Neutrophil Elastase, and TO-PRO-3 reveals hidden immune–tumor interactions and sets a new standard for research.

See an entire hair follicles in true 3D, from bulb to dermal papilla, with detailed mapping of sensory nerves and nuclei for alopecia and hair research.High-resolution 3D imaging of scalp tissue reveals nuclei, immune cells, and nerve networks, driving new insights into alopecia and other skin diseases.

3D imaging of atopic dermatitis skin punch biopsy; the tissue is stained with TO-PRO-3, PGP9.5, and CD45. Explore detailed innervation and immune-cell interactions in lesional skin.

From vasculature to single cells, 3D light-sheet imaging reveals the intricate architecture of a whole mouse brain in unparalleled detail.

3D imaging of mouse colorectal tumor stained with CD3, B220, and YoPro1 reveals tertiary lymphoid structures (TLS) in full spatial context.

3D fluorescence imaging of human lung tissue stained with Fast Green reveals collagen architecture for fibrosis and tissue remodeling research.

3D imaging of human skin biopsy stained with tryptase, TO-PRO-3, and PGP9.5 reveals mast cell–nerve interactions for dermatology and oncology research.

High-resolution 3D imaging of a cleared rabbit retina stained with TO-PRO-3 and eosin, preserving architecture across retinal, choroid, and scleral layers.

3D imaging of prostate organoids stained with TO-PRO-3 and eosin reveals spatial heterogeneity, cellular interactions, and microenvironmental detail.

High-resolution 3D imaging of a mouse pancreatic cyst stained with Ki67 and γ-H2AX reveals proliferation and DNA damage in full spatial context.

3D fluorescence imaging of prostate tissue stained with Fast Green reveals the collagen architecture, orientation, and density, providing insights beyond 2D histology.

High-resolution 3D imaging of human tonsil tissue stained with YO-PRO-1 for nuclei and tryptase for mast cells.

3D imaging of prostate organoids with LUMI shows ROI selection, multi-well scanning, and high-resolution analysis using TO-PRO-3 and eosin staining.

See the full Aurora Workflow in action with 3D imaging of murine heart vasculature, delivering vessel measurements, branching analysis, and cell counts.

LUMI (Light-Sheet User Microscope Interface) enhances HOTLS with effortless setup, live preview, and smart automation for seamless 3D imaging.

2 mm pig muscle section stained with CD31 and YO-PRO-1 imaged in 3D on Aurora HOTLS, revealing vascular networks with speed and precision.

3D imaging of human placenta tissue stained with SMA, HLA-G, and CD31. From whole-tissue scans to single-cell resolution, uncover tissue complexity in detail.

Whole mouse brain imaged in 3D with TO-PRO-3 and eosin. Scout and Zoom workflows capture glioblastoma ROI, segmentation, and high-resolution morphology.

Scout 3D imaging of a human DRG sample from AnaBios with computational H&E staining, ToPro-3 for nuclei, and eosin for protein structures.

Zoomed 3D imaging of human DRG sample from AnaBios with computational H&E staining, ToPro-3 for nuclei, and eosin for protein structures.

3D imaging of cleared liver tissue stained with Collagen III, followed by segmentation analysis using a pixel classifier to highlight fibrosis.

3D spatial biology image of liver biopsy segmented with AI, highlighting fibrosis (cyan) and steatosis (yellow) for quantitative pathology insights.

A 3D fly-through of liver tissue stained with computational hematoxylin and eosin, showcasing structures beyond traditional 2D pathology.

3D imaging of human colon stained with Calretinin reveals dense innervation of the mucosa and submucosa.

A 3D scan of a human ileocecal sample with over 840 billion pixels and 2,350 mm³ volume, imaged on Alpenglow’s Aurora HOTLS platform.

Large cleared human brain slice (10 × 7 × 0.3 cm) imaged at 0.17 microns per pixel using the CUBIC protocol for high-resolution volumetric analysis.

3D imaging of cleared tonsil tissue stained with anti-CD21 and TO-PRO-3 reveals B cell and follicular dendritic cell distribution in FFPE samples.

3D imaging of cleared tonsil tissue stained with anti-CD45RO (UCHL1) and TO-PRO-3 reveals memory T cell distribution in thick FFPE samples.

3D imaging of cleared tonsil tissue stained with anti-CD3 (BC33) and TO-PRO-3 reveals the distribution of T cells in thick FFPE samples on the Aurora HOTLS platform.

3D imaging of cleared tonsil tissue stained with anti-CD8 (SP6) and TO-PRO-3 reveals cytotoxic T cells in thick, FFPE samples imaged on the Aurora platform.

High-resolution 3D imaging of human tonsil reveals cytotoxic T cells and macrophages stained with CD8 and CD68, mapped in their native spatial context.

High-resolution 3D imaging of scalp epidermis reveals branching nerve structures and nuclear organization, advancing hair and dermatology research.

See hair follicles in true 3D, from bulb to dermal papilla, with detailed mapping of sensory nerves and nuclei for alopecia and hair research.High-resolution 3D imaging of scalp tissue reveals nuclei, immune cells, and nerve networks, driving new insights into alopecia and other skin diseases.

High-resolution 3D imaging of scalp tissue reveals nuclei, immune cells, and nerve networks, driving new insights into alopecia and other skin diseases.

High-resolution 3D imaging of atopic dermatitis reveals precise innervation and immune cell distribution, surpassing conventional histology.

High-resolution 3D imaging of CD45-stained skin biopsy shows lymphocyte clustering around nerves, offering new insights into immune cell behavior.

Journey through the delicate nerve structures of the epidermis and dermis at low resolution, and zoom in for an awe-inspiring close-up of lymphocyte distribution around these dynamic networks.

Low resolution image of a lesional Atopic Dermatitis sample stained with PGP9.5 (white) to highlight fine epidermal innervation, TO-PRO-3 (blue) for nuclear detail, and CD3 (green) to illuminate T-Cell infiltration of the Epidermis and Dermis.

40X image of a lesional Atopic Dermatitis samples stained with PGP9.5 (white) to highlight fine epidermal innervation, TO-PRO-3 (blue) for nuclear detail, and CD3 (green) to illuminate T-Cell infiltration of the Epidermis and Dermis.

In a Dynamk Capital interview, Alpenglow CEO Nick Reder, MD MPH, discusses 3D spatial biology, AI-powered analysis, and accelerating drug discovery.

3D fluorescence imaging of human colorectal cancer FFPE tissue reveals spatial organization across depth that cannot be captured with single 2D histology sections.