3D view of Human Normal Adjacent Colorectal Cancer

Written By Elisa Barbieri

Explore this cutting-edge 3D imaging of Human Normal Adjacent Colorectal Cancer:

  • Tissue Preparation: FFPE tissue was deparaffinized, processed, and stained with the nuclear dye YO-PRO-1 (blue) and an anti-CD8 antibody (yellow).

  • Optical Clearing: Modified iDISCO+.

  • Imaging: Achieved at a 2 μm/pixel resolution using the Hybrid Open-Top Light-Sheet (HOTLS) microscope from Alpenglow Biosciences (3Di).

  • Analysis: CD8+ lymphocytes were quantified across the entire tissue section (approximately 6 mm x 4.3 mm x 4.2 mm) using advanced 3D spatial analysis tools.

Elisa Barbieri
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