3D Imaging of Human Tonsil Tissue Highlighting Mast Cells with Tryptase Staining

Experience the power of 3D fluorescence imaging at Zoom resolution using Hybrid Open Top Light Sheet (HOTLS) microscopy.

This visualization of human tonsil tissue (~800 µm in length) captures intricate spatial relationships that traditional 2D imaging would miss:

• YO-PRO-1 staining for nuclei (purple),

• Tryptase for mast cells (cyan), a critical marker in allergic responses, inflammation, and tumor microenvironments.

With HOTLS microscopy, we achieve high-resolution imaging across large tissue volumes, providing a full-depth view of cellular organization in its native context. This level of detail is essential for understanding immune responses and disease pathology at a new scale.

Curious about the antibody used? This is from Abcam, Cat: ab2378, Clone: [AA1], https://www.abcam.com/en-us/products/primary-antibodies/mast-cell-tryptase-antibody[…]sltid=AfmBOoqottEqHLRMk43Xza7e0WYltzxI64-3fpUIGDxi0DXcz7tJhEA_